Thermo Fisher Scientific Bcl-6 Monoclonal Antibody (BCL6, 1718)
Human Bcl-6 단백질을 인식하는 Mouse monoclonal antibody로, IHC(P) 및 Peptide Array에 사용 가능. Recombinant Bcl-6 fragment(aa256-389)를 면역원으로 제작. Protein A/G 정제, PBS buffer에 200 µg/mL 농도. 연구용 시약으로 림프종 관련 연구에 적합.
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1–2 µg/mL
Peptide Array (Array)
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | BCL6, 1718 |
| Immunogen | Recombinant human Bcl-6 protein fragment (aa256–389) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Bcl-6 proto-oncogene product is a Kruppel-type zinc-finger protein mainly expressed in normal germinal center B cells and related lymphomas.
It is involved in chromosome rearrangements at 3q27 in non-Hodgkin’s lymphomas, detected in 33–45% of diffuse large B cell lymphomas.
Immunohistochemically, Bcl-6 has been found in follicular lymphomas, diffuse large B cell lymphomas, Burkitt’s lymphomas, and nodular lymphocyte predominant Hodgkin’s disease.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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