Thermo Fisher Scientific GAPDH Monoclonal Antibody (6C5)

Applications

Tested Dilution

Publications

Western Blot (WB)

3 µg/mL

View 650 publications 650 publications

Immunohistochemistry (IHC)

-

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1 µg/mL

View 8 publications 8 publications

Immunoprecipitation (IP)

-

View 2 publications 2 publications

Functional Assay (Functional)

-

View 1 publication 1 publication

Control (Ctrl)

-

View 1 publication 1 publication

Dot blot (DB)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 30 publications 30 publications

Product Specifications

Species Reactivity

Amphibian, Dog, Chicken, Fish, Human, Mouse, Non-human primate, Rabbit, Rat

Published species

Amphibian, Bovine, C. elegans, Chicken, Dog, Fruit fly, Hamster, Human, Insect, Mouse, Nematode, Non-human primate, Pig, Rat, Xenopus, Yeast, Zebrafish

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

6C5

Immunogen

Purified rabbit muscle GAPDH (whole molecule). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GAPDH,uniProtId:P46406-1,ncbiNodeId:9986,antigenRange:1-333,antigenLength:333,antigenImageFileName:AM4300_GAPDH_P46406-1_House_mouse.svg,antigenImageFileNamePDP:AM4300_GAPDH_P46406-1_House_mouse_PDP.jpeg,sortOrder:4}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

Shipping conditions

Dry ice

RRID

AB_2536381

Target Information

GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimers disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntingtons disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.