Invitrogen™

pMIR-REPORT™ miRNA Expression Reporter Vector System

The Ambion™ pMIR-REPORT™ miRNA Expression Reporter Vector System is for the measurement of miRNA expression in cells. It contains two자세히 알아보기

The Ambion™ pMIR-REPORT™ miRNA Expression Reporter Vector System is for the measurement of miRNA expression in cells. It contains two mammalian expression vectors—one for cloning miRNA targets and evaluating miRNA regulation, and the other for the normalization of transfection efficiency.

• Clone miRNA targets and evaluate miRNA regulation
• Screen putative miRNA target sequences
• Includes both reporter and control vectors

The pMIR-REPORT™ miRNA Expression Reporter Vector System provides accurate, quantitative, in-cell measurement of miRNA expression. This validated reporter system contains two mammalian expression vectors. The pMIR-REPORT™ Luciferase miRNA Expression Reporter Vector contains firefly luciferase under the control of a mammalian promoter/terminator system, with an miRNA target cloning region downstream of the luciferase translation sequence. This vector is optimized for cloning miRNA targets and evaluating miRNA regulation. A second vector, pMIR-REPORT™ Beta-galactosidase Reporter Control Vector, is provided for normalizing transfection efficiency.

Applications:
pMIR-REPORT™ Luciferase is designed for cloning and testing putative miRNA binding sites. pMIR-REPORT™ Luciferase can be transfected into mammalian cells to evaluate endogenous miRNA expression, or used to evaluate the up- or down-regulation resulting from the transfection of Pre-miR™ miRNA Molecules or Anti-miR™ miRNA Inhibitor Molecules, respectively.

사양

제품 유형RNAi Expression Vector Kit

유전자Beta-Gal (lacZ), Luciferase

클로닝 방법Restriction Enzyme ⁄ MCS

구성 또는 유도성 시스템Constitutive

배달 유형Transfection

RNAi 유형miRNA

선택 제제(진핵)Puromycin

배송 조건Dry Ice

벡터pMIR

프로모터CMV

제품라인Ambion™, pMIR-REPORT™

수량1 kit

Unit SizeEach