Thermo Fisher Scientific p16INK4a Recombinant Mouse Monoclonal Antibody (PD01-16)
Recombinant mouse monoclonal antibody targeting p16INK4a for WB, IHC, and ICC applications. High specificity and sensitivity for human samples. Supplied as liquid form, 1 mg/mL concentration, purified by Protein A, stored at 4°C or -20°C. For research ...
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Thermo Fisher Scientific p16INK4a Recombinant Mouse Monoclonal Antibody (PD01-16)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | PD01-16 |
| Immunogen | Synthetic peptide aa 1–126/156 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 0.1% BSA, 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C and avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3092897 |
Product Specific Information
- Positive Controls: Human tonsil tissue, cervix adenocarcinoma tissue, cervix squamous cell carcinoma tissue, ovary serous carcinoma tissue, HeLa, HepG2, HEK-293, NIH:OVCAR-3 cell lysates.
- Predicted Band Size: 16 kDa
- Subcellular Location: Cytoplasm, Nucleus
Target Information
This gene produces multiple transcript variants differing in their first exons. Two variants encode isoforms that inhibit CDK4 kinase, while another variant encodes an alternate reading frame (ARF) protein that stabilizes p53 by interacting with MDM2. Despite structural differences, all variants contribute to cell cycle G1 control through CDK4 and p53 regulation. The gene is frequently mutated or deleted in various tumors and functions as an important tumor suppressor.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
제품 이미지
(이미지 파일: MA5-50659_p16INK4a_P42771-1_House_mouse.svg, MA5-50659_p16INK4a_P42771-1_House_mouse_PDP.jpeg)
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