Thermo Fisher Scientific BACE1 Monoclonal Antibody (3C1C3)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:100

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Human, Mouse, Non-human primate, Rat

Published species

Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3C1C3

Immunogen

Purified recombinant fragment of Human BACE1 (AA: 112-324) expressed in E. Coli if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:BACE1,uniProtId:P56817-1,ncbiNodeId:9606,antigenRange:112-324,antigenLength:501,antigenImageFileName:MA1-177_BACE1_P56817-1_House_mouse.svg,antigenImageFileNamePDP:MA1-177_BACE1_P56817-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2608440

Product Specific Information

MA1-177 targets BACE1 in WB and IF, shows reactivity with Human, Primate, Canine, Mouse and Rat samples.

Target Information

BACE1 (beta-secretase) is a key enzyme involved in the production of amyloid beta-peptides (Abeta) found in extracellular amyloid plaques of Alzheimers disease (AD). In some cases early onset familial AD can be attributed to aSwedishmutation in the amyloid precursor protein (APP), which dramatically enhances the cleavage of this protein by BACE1. This and other genetic and pathological evidence has led to therapeutic approaches that have focused on the inhibition of BACE1 and other APP-cleaving enzymes, such as gamma-secretase stuff. APP is first cleaved by b-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for g-secretase to generate the 4 kDa amyloid-b peptide, which is deposited in the brains of all sufferers of Alzheimers disease. The long-sought b-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2). bACE/Asp2 is a novel transmembrane aspartic protease and colocalizes with APP.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.