Thermo Fisher Scientific GM130 Polyclonal Antibody
Rabbit polyclonal antibody targeting human GM130 protein for use in WB, IHC, and IP applications. High specificity and affinity purified via antigen chromatography. Supplied in liquid form, stored at 4°C, recommended shelf life of 1 year.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 952 and 1002 of human 130 kDa cis-Golgi Matrix Protein (GM130) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC: Epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
Target Information
The Golgi apparatus participates in glycosylation and transport of proteins and lipids in the secretory pathway and consists of stacked cisternae. Interactions between the Golgi and microtubules are crucial for its reorganization during mitosis. GM130 is one of the golgins, a family of proteins localized to the Golgi, and is implicated in Golgi stacking and vesicular transport. Several alternatively spliced transcript variants exist, though their full-length nature remains undetermined.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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