Thermo Fisher Scientific CD163 Monoclonal Antibody (TNKUPJ), PE, eBioscience
Mouse CD163 인식을 위한 TNKUPJ 단클론 항체로, PE 형광체가 결합된 제품입니다. Flow cytometry 및 IHC에 사용 가능하며, 고순도 친화 크로마토그래피 정제. 565/576 nm 형광 특성, 4°C 암저장 권장.
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Thermo Fisher Scientific CD163 Monoclonal Antibody (TNKUPJ), PE, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | View 17 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TNKUPJ |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2716923 |
Available Formats
- Unconjugated
- Alexa Fluor 532
- APC
- Brilliant UV 615
- Brilliant UV 661
- Brilliant UV 737
- Brilliant UV 805
- Brilliant Violet 421
- eFluor 450
- FITC
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 702
- Request custom conjugation
Product Specific Information
This TNKUPJ monoclonal antibody recognizes mouse CD163, a 130 kDa surface receptor expressed by specific tissue macrophages such as splenic red pulp macrophages, Kupffer cells, intestinal lamina propria macrophages, and a small fraction of peritoneal macrophages. Mouse monocytes do not express CD163, and unlike human CD163, it is not a reliable M2 macrophage marker.
This antibody can detect CD163 in fixed and permeabilized cells, enabling intracellular staining. For tissues subjected to aggressive dissociation, intracellular detection is recommended.
Applications Reported: Flow cytometric analysis.
Applications Tested: Flow cytometric analysis of mouse splenocytes at ≤0.25 µg/test (100 µL final volume). Optimal titration recommended.
Excitation: 488–561 nm; Emission: 578 nm; Compatible Lasers: Blue, Green, Yellow-Green.
Target Information
CD163 (M130 antigen, Ber-Mac3, Ki-M8, SM4) is a 130 kDa membrane glycoprotein and member of the scavenger receptor cysteine-rich superfamily. It acts as a receptor for the hemoglobin-haptoglobin complex, protecting tissues from oxidative damage and participating in iron recycling.
CD163 is expressed on human monocytes and macrophages, upregulated by IL-10, IL-6, and dexamethasone, and shed upon LPS or PMA stimulation. It binds hemoglobin/haptoglobin complexes in a calcium- and pH-dependent manner, initiating intracellular signaling cascades involving tyrosine kinase-dependent calcium mobilization and secretion of IL6 and CSF1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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