Thermo Fisher Scientific BCL-w Polyclonal Antibody
BCL-w 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal 항체로, Western Blot 및 IHC(Paraffin)에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, PBS 완충액과 안정제가 포함된 액상 형태로 제공됩니다. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Dog, Chicken, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide derived from N-terminus of human BCL-W protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Breast carcinoma
- Cellular location: Mitochondria outer membrane, Nucleus membrane
Target Information
Using a PCR-based strategy, an additional protein with life-promoting activity, designated Bcl-w, has been identified.
The protein is highly conserved between mouse and human and is encoded by a gene located near the TCRα gene on chromosome 14.
Bcl-w is expressed in myeloid cell lines but not in T and B lymphocytes, and can be found in a wide range of tissues.
An alternative splicing event in exon 4 results in two transcripts:
- Bcl-w: encodes a protein of 193 amino acids
- Bcl-w/rox: encodes a protein 333 amino acids in length
The rox portion of Bcl-w/rox shows 66% amino acid sequence identity with the Drosophila rox2 protein; however, the Bcl-w/rox transcript may be expressed at very low levels.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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