Takara DNA extraction from FFPE samples

DEXPAT reagents are designed for one-step extraction of DNA from paraffin-embedded tissues fixed with 10% formalin (FFPE). The time needed for preparing PCR-ready DNA from paraffin-embedded tissue is dramatically reduced to 25 minutes from the 2–3 days required when using conventional methods.

DEXPAT reagents are designed for one-step extraction of DNA from paraffin-embedded tissues fixed with 10% formalin (FFPE). The time needed for preparing PCR-ready DNA from paraffin-embedded tissue is dramatically reduced to 25 minutes from the 2–3 days required when using conventional methods. To obtain PCR-ready DNA, simply add a paraffin-embedded tissue section to a tube containing DEXPAT Reagent or DEXPAT Easy, incubate it at 100°C, centrifuge at 4°C, and allow to cool on ice for 5 minutes. The refrigerated centrifugation step following heat treatment allows the formation of a gel-like layer above the absorbent resin. When collecting the DNA solution, this gel-like layer prevents contamination of the DNA by the absorbent resin.

DEXPAT Easy retains the performance of DEXPAT Reagent while offering the additional benefits of a ready-to-use reagent dispensed into individual microcentrifuge tubes, as well as an improved composition that facilitates separation of the aqueous DNA solution from the included absorbent resin.

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