Thermo Fisher Scientific NFIX Polyclonal Antibody

Applications

Immunohistochemistry (IHC)

• Tested Dilution: Not specified
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Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:100–1:1,000
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Product Specifications

Product Specific Information

• Recommended positive control: U87-MG
• Predicted reactivity: Mouse (99%), Xenopus laevis (89%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

NF-1 (also designated CTF) is a family of CCAAT box binding proteins that stimulate DNA replication and activate transcription. Human NF-1 mRNA analysis reveals two forms arising from alternative splicing of a single gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA-binding domain and activates transcription through a proline-rich, carboxy-terminal transactivation domain. NF-1 recognizes and binds the adenovirus type 2 promoter and activates transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element is found in upstream promoter regions of various eukaryotic genes, including Ha-Ras, alpha-globin, HSP70, GRP78, Histone H1, myelin basic protein, and Xenopus laevis vitellogenin gene promoter.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.