Thermo Fisher Scientific iNOS Monoclonal Antibody (CXNFT), APC, eBioscience
마우스 iNOS 단백질을 검출하는 CXNFT 클론의 Rat IgG2a 단일클론 항체로, APC 형광체가 결합되어 있습니다. 유세포 분석에 최적화되어 있으며, 0.06 µg/test로 사용 권장됩니다. 651/660 nm 파장에서 작동하며, 4°C 암소 보관이 필요합니다.
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Thermo Fisher Scientific iNOS Monoclonal Antibody (CXNFT), APC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.06 µg/test | View 48 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CXNFT |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573243 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 532
- Alexa Fluor 700
- APC-eFluor 780
- Brilliant UV 395
- Brilliant UV 563
- Brilliant UV 615
- Brilliant UV 737
- Brilliant Violet 421
- Brilliant Violet 480
- Brilliant Violet 605
- Brilliant Violet 786
- eFluor 450
- PE
- PE-Cyanine5.5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
This CXNFT monoclonal antibody reacts to mouse NOS2 (inducible NOS, iNOS). Nitric oxide synthase enzymes catalyze the formation of nitric oxide from L-arginine through an NADPH- and oxygen-dependent mechanism. NOS2 is induced in response to bacterial endotoxins and inflammatory cytokines such as IFN-γ and TNF-α, and is expressed by myeloid-derived suppressor cells and M1 macrophages.
Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Intracellular staining and flow cytometric analysis of LPS-stimulated mouse thioglycolate-elicited peritoneal exudate cells using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00). Recommended usage ≤ 0.06 µg/test (100 µL final volume).
Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
iNOS (Inducible Nitric Oxide Synthase) is an enzyme that catalyzes the production of nitric oxide (NO) from L-arginine. It is expressed in various cell types such as macrophages, hepatocytes, and smooth muscle cells, and is inducible by cytokines (IFN-γ, TNF, IL-1/IL-2) and lipopolysaccharides (LPS). iNOS plays roles in vasorelaxation, neurotransmission, and immune response. Dysregulation of iNOS is associated with diseases such as achalasia and impotence.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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