Thermo Fisher Scientific Human CRTH2 Synthetic Peptide

Applications

Tested Dilution

Publications

Control (Ctrl)

Assay-dependent

-

Blocking Assay (BLOCK)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Class

Synthetic

Type

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

200 µg/mL

Purification

purified

Storage buffer

PBS, pH 7.2, with 0.1% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

This peptide corresponds to 15 amino acid peptide from near the center of human CRTH2

PEP-0453 can be used as a blocking peptide with polyclonal antibody PA5-20333.

Target Information

CRTH2 (GPR44) is a chemoattractant receptor-homologous molecule expressed on Th2 cells that is an identified receptor for the prostaglandin D2 (PGD2) and prostaglandin D receptor. CRTH2 is expressed on Th2 cells and eosinophils and mediates chemotaxis of these cells to PGD2 and is thus thought to be a key receptor mediating eosinophil and Th2 recruitment during allergic responses. However, CRTH2- mice showed enhanced eosinophil recruitment into the lung consistent with observations that the CRTH2- mice produced significantly higher amounts of interleukin-5 (IL-5) and IL-3. This suggests that CRTH2 plays a nonredundant role in restricting eosinophilia and allergic response in vivo. CRTH2 is also a novel member of the G protein-coupled leukocyte chemoattractant receptor family, which is selectively expressed in Th2 but not Th1 lineage cells. CRTH2 has been reported to be expressed in the central nervous system, peripheral tissues, and in blood cells, particularly activated T helper-2 cells. Two transcripts have been isolated: a 3.5 kb transcript expressed primarily in the thalamus, frontal cortex, pons and hippocampus; and a 3.4 kb transcript expressed in fetal liver, leukocytes and thymus. CRTH2 is selectively expressed in vivo in an activated state of Th2 cells including allergen-responsive Th2 cells, suggesting its pivotal roles in ongoing Th2-type immune reactions. At least two different isoforms of CRTH2 are known to exist. Single nucleotide polymorphisms in the 3` UTR of the CRTH2 gene have been associated with asthma susceptibility.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.