
Thermo Fisher Scientific EIF4A1/EIF4A2/EIF4A3 Polyclonal Antibody
EIF4A1/EIF4A2/EIF4A3 폴리클로날 항체로 인간, 생쥐, 랫트 시료에 반응. WB, ICC/IF, ELISA에 적합. 합성 펩타이드 면역원 기반, 친화 크로마토그래피로 정제. 고농도 액상 형태, 안정적 저장(-20°C) 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1–100 of human eIF4A (NP_0014071) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.638 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2901580 |
Product Specific Information
- Positive Samples: 293T, HeLa, HepG2, MCF7, C2C12, Mouse brain, Rat brain
- Immunogen sequence:
MSASQDSRSR DNGPDGMEPE GVIESNWNEI VDSFDDMNLS ESLLRGIYAY GFEKPSAIQQ RAILPCIKGY DVIAQAQSGT GKTATFAISI LQQIELDLKA
Target Information
The eIF4A proteins are involved in many aspects of RNA metabolism due to their RNA-binding capacity and ATPase activity.
eIF4A1 and eIF4A2 are cytoplasmic, whereas eIF4A3 is nuclear-localized.
In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs, allowing efficient binding of the small ribosomal subunit and subsequent scanning for the initiator codon.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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