Thermo Fisher Scientific EIF4A1/EIF4A2/EIF4A3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human eIF4A (NP_0014071) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:EIF4A1/EIF4A2/EIF4A3,uniProtId:P38919-1,ncbiNodeId:9606,antigenRange:1-100,antigenLength:411,antigenImageFileName:PA5-116950_EIF4A1EIF4A2EIF4A3_P38919-1_Rabbit.svg,antigenImageFileNamePDP:PA5-116950_EIF4A1EIF4A2EIF4A3_P38919-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:EIF4A1/EIF4A2/EIF4A3,uniProtId:P60842-1,ncbiNodeId:9606,antigenRange:1-100,antigenLength:406,antigenImageFileName:PA5-116950_EIF4A1EIF4A2EIF4A3_P60842-1_Rabbit.svg,antigenImageFileNamePDP:PA5-116950_EIF4A1EIF4A2EIF4A3_P60842-1_Rabbit_PDP.jpeg,sortOrder:2},{targetFamily:EIF4A1/EIF4A2/EIF4A3,uniProtId:Q14240-1,ncbiNodeId:9606,antigenRange:1-100,antigenLength:407,antigenImageFileName:PA5-116950_EIF4A1EIF4A2EIF4A3_Q14240-1_Rabbit.svg,antigenImageFileNamePDP:PA5-116950_EIF4A1EIF4A2EIF4A3_Q14240-1_Rabbit_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.638 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2901580

Product Specific Information

Positive Samples: 293T, HeLa, HepG2, MCF7, C2C12, Mouse brain, Rat brain

Immunogen sequence: MSASQDSRSR DNGPDGMEPE GVIESNWNEI VDSFDDMNLS ESLLRGIYAY GFEKPSAIQQ RAILPCIKGY DVIAQAQSGT GKTATFAISI LQQIELDLKA

Target Information

The eIF4A proteins are involved in many aspects of RNA metabolism due to their RNA-binding capacity and ATPase activity. eIF4A1 and eIF4A2 are cytoplasmic, whereas eIF4A3 is nuclear-localized. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5`-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.