Thermo Fisher Scientific RFC2 Monoclonal Antibody (OTI3A7), TrueMAB
RFC2 단백질을 인식하는 마우스 단일클론 항체로, Western blot, IHC, ICC, Flow cytometry에 사용 가능. 정제된 Lyophilized 형태로 제공되며, 재구성 후 1 mg/mL 농도로 사용. 인간 단백질에 반응하며 연구용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3A7 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–234 of human RFC2 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step.
Recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
The RFC2 gene encodes a member of the activator 1 small subunits family. DNA elongation by polymerases δ and ε requires accessory proteins such as proliferating cell nuclear antigen (PCNA) and replication factor C (RFC).
RFC, also known as activator 1, is a complex of five distinct subunits. The 40 kDa subunit encoded by this gene binds ATP and contributes to cell survival.
Disruption of this gene is associated with Williams syndrome. Alternative splicing generates distinct isoforms, and a pseudogene exists on chromosome 2.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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