Thermo Fisher Scientific HLA-DRw52 Monoclonal Antibody (7.3.19.1)

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

1:10

-

Cytotoxicity Assay (CT)

1:10

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

7.3.19.1

Immunogen

Human HLA-DRw52

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

PBS with 0.1% BSA

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_1076829

Product Specific Information

The antibody reacts with a polymorphic determinant on HLA-DR molecules which is associated with DR3, DR5, and DRw6 (DRw52-like). It does not react with DRw8. The antibody reacts with part of the HLA-D molecules (29+34 kD). The determinant is strongly expressed on DR3 positive cells and more weakly on DR5 and DRw6 positive cells. The antibody is useful for HLA-DR typing and biochemical characterization of the DR52w positive molecules and functional studies. Cross reacts with Chimpanzee, Macaca-apes and gorilla.

Target Information

HLA DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of an alpha chain (36 kDa) and a beta chain (27 kDa). It is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They therefore have a critical role in the initiation of the immune response.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.