Thermo Fisher Scientific IFN alpha Monoclonal Antibody (2-48)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2-48

Immunogen

E.coli derived recombinant human interferon alpha 1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

purified

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2248769

Product Specific Information

The alpha interferons are involved in virus resistance in target cells for these viruses. They are known to block cell proliferation and to regulate MHC class I antigen expression. The IFNα family has over 20 genes and pseudogenes in two families (I and II), one with a mature length of 166aa and one of 172aa. Cells producing IFNα are lymphocytes, monocytes, macrophages and cell lines such as Namalwa and KGI. Bioassays for IFNα include cytopathic effect blocking, by viruses such as VSV, SFV and BMCV, on their target cells. A number of receptors for IFNα are now known and seem to be expressed on most cell types. 2-48 Is specific for human interferon α 1 and does not cross react with human interferon α 2.

Target Information

IFN alpha (Interferon alpha) is a Type I interferon belonging to a family of structurally related, species-specific proteins exclusive to vertebrates. The interaction of IFN-alpha with its receptor component result in the activation of a number of signaling pathways which are mainly involved in innate immune response against viral infection. In humans, the gene for IFN alpha is located on on chromosome 9. The type I interferons consist of 14 different alpha isoforms (subtypes with slightly different specificities), and single beta, omega, epsilon and kappa isoforms. Homologous molecules are found in many species, including rats and mice (and most mammals), and have been identified in birds, reptiles and fish species. IFN alpha is produced primarily by plasmacytoid dendritic cells, is a potent component of the anti-viral innate immune response and modulates adaptive immunity. Diseases associated with the dysfunction of IFN alpha include Hepatitis C and Hepatitis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.