
Thermo Fisher Scientific HCN4 Polyclonal Antibody
HCN4 단백질을 인식하는 Thermo Fisher Scientific의 기니피그 유래 폴리클로날 항체. Western blot 및 동결 조직 면역염색에 적합. 항원 친화 크로마토그래피로 정제되었으며, PBS 버퍼에 1% BSA 포함. 심장 및 신경계 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:200 | - |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Guinea pig / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GST fusion protein with the sequence HGHLHDSAEE RRLIAEGDASPGEDRTPPGLAAEPERP, corresponding to amino acid residues 119–155 of human HCN4 (Intracellular, N-terminus) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.8 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitution:
Reconstitute with 25 µL, 50 µL, or 0.2 mL of double distilled water (DDW), depending on sample size.
The antibody is shipped as a lyophilized powder at room temperature.
Upon arrival, store at −20°C.
The reconstituted solution can be stored at 4°C for up to 1 week.
For long-term storage, aliquot and store at −20°C.
Avoid multiple freeze/thaw cycles.
Centrifuge all antibody preparations before use (10,000 × g, 5 min).
Target Information
HCN4 is a member of the hyperpolarization-activated and cyclic-nucleotide-gated (HCN) channel family.
HCN currents are linked to pacemaker activity in the heart and brain, resting potential control, and neuronal plasticity.
HCN4 channels function as receptors for sour taste and are associated with pacemaker potential generation in the sinoatrial node.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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