Thermo Fisher Scientific Aurora A Monoclonal Antibody (35C1)
Aurora A 단백질을 인식하는 마우스 모노클로날 항체로, Western blot, ICC/IF, IP, ChIP 등 다양한 응용에 사용 가능. 비촉매 N-말단 도메인 특이적 인식. 암 연구 및 세포주 분석용 연구용 시약.
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Applications and Tested Dilution
| Application | Tested Dilution / Conditions |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Immunoprecipitation (IP) | Assay-dependent |
| ChIP assay (ChIP) | 1–5 µg |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 35C1 |
| Immunogen | Recombinant Aurora A |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C or -20°C if preferred |
| Shipping Conditions | Wet ice |
| RRID | AB_2061334 |
Product Specific Information
Clone 35C1 specifically recognizes an epitope within the non-catalytic N-terminal domain of Aurora A and does not inhibit Aurora A kinase activity.
A suggested positive control for immunohistochemical applications is human 293 and mouse llc1 cell lines.
In Western blot, this antibody detects a band at approximately 46 kDa in human HeLa and mouse M-ICc12 cell lysates.
Target Information
AURKA (Aurora A) is a serine/threonine kinase that regulates mitosis through association with centrosomes.
Overexpression of AURKA is observed in many types of cancer. Aurora A plays a role in cell cycle regulation during anaphase and/or telophase, particularly in the centrosome/spindle pole region during chromosome segregation.
Aurora A is a key factor in tumor development and progression and is overexpressed in multiple human cancers including breast, ovarian, and colorectal cancers.
Tissue specificity: Highly expressed in testis and weakly in skeletal muscle, thymus, and spleen.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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