Thermo Fisher Scientific Aurora A Monoclonal Antibody (35C1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

ChIP assay (ChIP)

1-5 µg

-

Product Specifications

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

35C1

Immunogen

Recombinant Aurora A

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4°C or -20°C if preferred

Shipping conditions

Wet ice

RRID

AB_2061334

Product Specific Information

Clone 35C1 specifically recognizes an epitope within the non-catalytic N-terminal domain of Aurora-A, and does not inhibit Aurora-A kinase activity.

A suggested positive control for immunohistochemical applications is human 293 and mouse llc1 cell lines. In Western blot, this antibody detects a band at ~46kDa in human HeLa and mouse M-ICc12 cell lysates.

Target Information

AURKA (Aurora A) is a serine/threonine kinase that regulates mitosis by association in vivo with centrosomes. Overexpression of AURKA is observed in many types of cancer. Aurora A plays a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. Aurora A plays a key role during tumor development and progression and is overexpressed in many human cancers including breast, ovarian and colorectal. Aurora A is viewed as a potential target for anticancer drug treatment. Tissue specificity: Highly expressed in testis and weakly in skeletal muscle, thymus and spleen.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.