Thermo Fisher Scientific MYLK Polyclonal Antibody, MaxPab

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

in situ PLA (PLA)

1:1200

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MYLK (ENSP00000355024, 1 a.a. approximately 992 a.a) full-length human protein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MYLK,uniProtId:Q15746-1,ncbiNodeId:9606,antigenRange:1-1914,antigenLength:1914,antigenImageFileName:H00004638-D01P_MYLK_Q15746-1_Rabbit.svg,antigenImageFileNamePDP:H00004638-D01P_MYLK_Q15746-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: MDFRANLQRQ VKPKTVSEEE RKVHSPQQVD FRSVLAKKGT SKTPVPEKVP PPKPATPDFR SVLGGKKKLP AENGSSSAET LNAKAVESSK PLSNAQPSGP LKPVGNAKPA ETLKPMGNAK PAETLKPMGN AKPDENLKSA SKEELKKDVK NDVNCKRGHA GTTDNEKRSE SQGTAPAFKQ KLQDVHVAEG KKLLLQCQVS SDPPATIIWT LNGKTLKTTK FIILSQEGSL CSVSIEKALP EDRGLYKCVA KNDAGQAECS CQVTVDDAPA SENTKAPEMK SRRPKSSLPP VLGTESDATV KKKPAPKTPP KAAMPPQIIQ FPEDQKVRAG ESVELFGKVT GTQPITCTWM KFRKQIQESE HMKVENSENG SKLTILAARQ EHCGCYTLLV ENKLGSRQAQ VNLTVVDKPD PPAGTPCASD IRSSSLTLSW YGSSYDGGSA VQSYSIEIWD SANKTWKELA TCRSTSFNVQ DLLPDHEYKF RVRAINVYGT SEPSQESELT TVGEKPEEPK DEVEVSDDDE KEPEVDYRTV TINTEQKVSD FYDIEERLGS GKFGQVFRLV EKKTRKVWAG KFFKAYSAKE KENIRQEISI MNCLHHPKLV QCVDAFEEKA NIVMVLEIVS GGELFERIID EDFELTEREC IKYMRQISEG VEYIHKQGIV HLDLKPENIM CVNKTGTRIK LIDFGLARRL ENAGSLKVLF GTPEFVAPEV INYEPIGYAT DMWSIGVICY ILVSGLSPFM GDNDNETLAN VTSATWDFDD EAFDEISDDA KDFISNLLKK DMKNRLDCTQ CLQHPWLMKD TKNMEAKKLS KDRMKKYMAR RKWQKTGNAV RAIGRLSSMA MISGLSGRKS STGSPTSPLN AEKLESEEDV SQAFLEAVAE EKPHVKPYFS KTIRDLEVVE GSAARFDCKI EGYPDPEVVW FKDDQSIRES RHFQIDYDED GNCSLIISDV CGDDDAKYTC KAVNSLGEAT CTAELIVETM EEGEGEGEEE EE

Target Information

MYLK (MLCK) is a serine/threonine kinase that phosphorylates myosin regulatory light chains to assist in the production of contractile activity (myosin-actin interaction). MLCK is responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.