Thermo Fisher Scientific IL-21 Human ProcartaPlex Simplex Kit

Invitrogen™ IL-21 Human ProcartaPlex™ Simplex Kit

The Human IL-21 Simplex ProcartaPlex Kit measures IL-21 protein and is designed to be combinable with other Simplex kits to create custom multiplex panels utilizing Luminex xMAP technology for protein detection and quantitation. When combining multiple Simplex kits (not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is required per assay plate regardless of plex size.

About ProcartaPlex Assays for the Luminex Platform

ProcartaPlex immunoassays are based on the sandwich ELISA principle, using two highly specific antibodies binding to different epitopes of one protein to quantify multiple protein targets simultaneously using a Luminex instrument.

• More results per sample: Measure multiple protein targets simultaneously in a single 25–50 μL sample
• Well-established Luminex technology: Highly referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 μL sample from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures identifiable by Luminex xMAP detection systems (e.g., Luminex 200, FLEXMAP 3D, MAGPIX). Similar to a sandwich ELISA, matched antibody pairs identify the protein of interest. Each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are detected with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE).

Unlike traditional ELISA, in ProcartaPlex assays the capture antibody is conjugated to a bead rather than adsorbed to a microplate well, allowing reagents to remain free-floating in solution. The Luminex instrument uses dual lasers—one to distinguish bead signatures and another to quantify RPE fluorescence proportional to protein concentration.

ProcartaPlex multiplex assays enable profiling of more target proteins using significantly less sample in the same time required for a traditional ELISA. Panels are available for multiple species (human, mouse, rat, nonhuman primate, porcine, and canine).

For more information, visit thermofisher.com/procartaplex.

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