Thermo Fisher Scientific RCC1 Polyclonal Antibody
Rabbit polyclonal antibody recognizing RCC1 (RanGEF) protein. Validated for WB, IHC, ICC/IF, and IP. Suitable for human and Xenopus samples. Supplied as liquid, unconjugated form. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution | References |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | - |
| Immunocytochemistry (ICC/IF) | Assay-dependent | - |
| Immunoprecipitation (IP) | Assay-dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | His-tagged recombinant Xenopus RCC1 protein purified from E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2179591 |
Product Specific Information
PA1-5868 detects RanGEF in human and Xenopus samples.
It has been successfully used in immunofluorescence, immunoprecipitation, immunohistochemistry, and Western blot applications.
The immunogen is His-tagged recombinant Xenopus RCC1 protein purified from E. coli.
Target Information
RCC1 (Ran guanine nucleotide exchange factor) is a guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP for GTP.
It regulates the onset of chromosome condensation during the S phase, binds nucleosomes and double-stranded DNA, and functions as part of a signal pathway detecting unreplicated DNA.
The RCC1-Ran complex plays a key role in nucleo-cytoplasmic transport, mitosis, and nuclear-envelope assembly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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