Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Secondary Antibody, Biotin
Goat anti-Mouse IgG (H+L) Biotin 결합형 2차 항체로, Western blot, ICC/IF, Flow cytometry, ELISA 등 다양한 면역분석에 사용 가능. 고순도 Affinity 정제 및 높은 민감도 보장. PBS/BSA 완충액 내 보존, 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:20,000 |
| Flow Cytometry (Flow) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2534743 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Additional Formats Available
- Agarose
- Alexa Fluor 488
- Alexa Fluor 488-10 nm colloidal gold
- Alexa Fluor 555
- Alexa Fluor 647
- Alexa Fluor 680
- Alexa Fluor 790
- Alexa Fluor Plus 405
- Alexa Fluor Plus 488
- Alexa Fluor Plus 647
- AP
- bGal
- DyLight 350
- DyLight 405
- DyLight 488
- DyLight 550
- DyLight 594
- DyLight 633
- DyLight 650
- DyLight 680
- DyLight 800 4X PEG
- FITC
- HRP
- PE
- Rhodamine
- TRITC
- WesternDot 625
- Custom conjugation available upon request
Product Specific Information
Each lot of this antibody is validated for sensitivity using ELISA and for specificity by immunoelectrophoresis (IEP).
Target Information
Anti-Mouse secondary antibodies are affinity-purified with well-characterized specificity for mouse immunoglobulins.
They are useful for detection, sorting, or purification of target proteins.
Secondary antibodies enable versatile detection systems (e.g., HRP, AP, fluorescence) and enhance signal sensitivity through amplification, as multiple secondaries can bind to a single primary antibody.
Typically, these antibodies are generated by immunizing the host animal with pooled mouse immunoglobulins and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation) to achieve high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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