Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Secondary Antibody, AP
Thermo Fisher Scientific의 Goat anti-Mouse IgG (H+L) Secondary Antibody, AP는 Western blot, IHC, ELISA에 적합한 고감도 폴리클로날 항체입니다. AP로 결합되어 신호 증폭이 우수하며, 친화 크로마토그래피로 정제되어 높은 특이성과 재현성을 제공합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | Alkaline Phosphatase (AP) |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 30 mM triethanolamine, 1% BSA, 5 mM MgCl₂, 0.1 mM ZnCl₂ |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2534742 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
Each lot’s sensitivity is confirmed using ELISA, and specificity is verified by immunoelectrophoresis (IEP).
Target Information
Anti-Mouse secondary antibodies are affinity-purified with well-characterized specificity for mouse immunoglobulins. They are useful for detection, sorting, or purification of mouse IgG targets. Secondary antibodies provide versatility for multiple detection systems (e.g., HRP, AP, fluorescence) and enhance sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody. These antibodies are typically generated by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to produce highly specific reagents.
Additional Formats Available
- Agarose
- Alexa Fluor 488
- Alexa Fluor 555
- Alexa Fluor 647
- Alexa Fluor 680
- Alexa Fluor 790
- Alexa Fluor Plus 405
- Alexa Fluor Plus 488
- Alexa Fluor Plus 647
- β-Gal
- Biotin
- DyLight 350, 405, 488, 550, 594, 633, 650, 680, 800 4X PEG
- FITC
- HRP
- PE
- Rhodamine
- TRITC
- WesternDot 625
- Custom conjugation available upon request
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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