Thermo Fisher Scientific VPS34 Polyclonal Antibody
VPS34 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, ICC/IF, IP에 사용 가능. Human, Mouse, Pig, Rat에 반응하며, 항원 친화 크로마토그래피로 정제됨. PBS/BSA/glycerol 완충액에 보관되며, 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100 | - |
| Immunoprecipitation (IP) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Pig, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide made to an internal portion (within residues 700–850) of human VPS34 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 0.1% BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2283904 |
Product Specific Information
- The target sequence has 87% sequence homology with Xenopus.
- Suggested positive control: HepG2 whole cell lysates, antigen standard for PIK3C3 (transient overexpression lysate).
Target Information
PIK3C3 (hVPS34) is the sole class III member of the PI3 Kinase family.
PIK3C3 phosphorylates phosphatidylinositol (PI) to produce phosphatidylinositol 3-phosphate [PI(3)P], which recruits downstream effectors containing FYVE and Phox domains to membranes.
PIK3C3 is involved in coupling nutrient signaling to mTOR and also plays a role in autophagy and intracellular trafficking. It is required for the abscission step in cytokinesis and for the transport of lysosomal enzyme precursors to lysosomes, mediating transport from early to late endosomes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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