Thermo Fisher Scientific UFD1L Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:100–1:500

Product Specifications

Target Information

Ubiquitin-mediated proteolysis requires the transfer of ubiquitin (Ub) to lysine groups on selected cellular proteins, which then promotes their degradation by the 26S proteasome. Ub-fusions are cleaved by Ub-specific processing proteases (UBps) or by the Ub-fusion degradation (UFD) pathway. The UFD pathway preferentially cleaves Ub-conjugated proteins with amino acid substitutions at the C-terminal glycine residue of Ub.
UFD1 protein, first identified in yeast S. cerevisiae, has a human homolog named UFD1L. In vitro, UFD1 attenuates degradation of Ub-fusions containing proline or valine substitutions at Gly76 by promoting multiubiquitination of the Ub chain. Mutations in the UFD1 gene are linked to CATCH22 syndrome, characterized by cardiac defects, cleft palate, and hypocalcemia, suggesting a role of this pathway in developmental processes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.