Thermo Fisher Scientific Prolactin Receptor Monoclonal Antibody (U5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 15 publications 15 publications

Immunohistochemistry (IHC)

-

View 11 publications 11 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

5 µg/mL

View 6 publications 6 publications

Flow Cytometry (Flow)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

View 2 publications 2 publications

Neutralization (Neu)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Horse, Human, Mouse, Pig, Rabbit, Rat

Published species

Baboon, Bovine, Hamster, Human, Mouse, Non-human primate, Pig, Rabbit, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

U5

Immunogen

Purified rat liver PRL receptor. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Prolactin Receptor,uniProtId:P05710-1,ncbiNodeId:10114,antigenRange:1-610,antigenLength:610,antigenImageFileName:MA1-610_Prolactin_Receptor_P05710-1_House_mouse.svg,antigenImageFileNamePDP:MA1-610_Prolactin_Receptor_P05710-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2171291

Product Specific Information

MA1-610 detects prolactin (PRL) receptor from rat, human, rabbit, horse, and pig tissues.

MA1-610 has been successfully used in Western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunofluorescence, and FACS procedures. By Western blot, this antibody detects an ~40 kDa protein and an ~100 kDa protein representing both the short and long forms of the PRL receptor, respectively, in various tissues. Immunohistochemical staining of PRL receptor in rat Islets of Langerhans with MA1-610 results in a staining pattern consistent with cytoplasmic vesicle staining. MA1-610, after biotinylation, has been used to detect human lymphocyte PRL receptor by FACS. MA1-610 does not inhibit the interaction of prolactin with the receptor.

The MA1-610 immunogen is purified PRL receptor from rat liver. This antibody has been shown to react with the extracellular portion of the receptor, but not in the ligand binding domain. MA1-610 does not inhibit the interaction of prolactin with the receptor.

Reconstitute with 100 µL PBS.

Target Information

Prolactin (PRL) is a pituitary hormone involved in the stimulation of milk production, salt and water regulation, growth, development and reproduction. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor (PRLR), a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. PRLR consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.