Thermo Fisher Scientific ASK1 Polyclonal Antibody
ASK1 단백질을 인식하는 Thermo Fisher Scientific의 폴리클로날 항체로, Western blot, IHC, ICC/IF 등에 사용 가능. 인간 및 마우스 반응성. 고순도 친화 크로마토그래피 정제. 장기 보관 시 -20°C 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide of C-terminal human ASK1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 0.2% BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2902982 |
Target Information
MAP3K5 (ASK1) is a serine/threonine kinase abundantly expressed in human heart and pancreas, activated in response to various extracellular stimuli such as cytokines, growth factors, and environmental stresses.
ASK1 (Apoptosis Signal-Regulating Kinase 1), also known as MAPKKK5, activates MAPKKs MKK4 and MKK6, which in turn activate JNK and p38 MAP kinases, respectively.
ASK1/MAPKKK5 is activated by TNFR and Fas through interactions with TRAF family members and the Fas-associated protein Daxx. Overexpression of ASK1 induces apoptotic cell death, while a catalytically inactive form inhibits TNF-α-induced apoptosis. ASK1 is expressed in various human and mouse tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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