Thermo Fisher Scientific Cytokeratin 19 Monoclonal Antibody (4F12G9)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunocytochemistry (ICC/IF)

1:100

-

ELISA (ELISA)

1:10,000

-

Product Specifications

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

4F12G9

Immunogen

Purified recombinant fragment of Cytokeratin 19 (aa80-400) expressed in E. Coli strain. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Cytokeratin 19,uniProtId:P08727-1,ncbiNodeId:9606,antigenRange:80-400,antigenLength:400,antigenImageFileName:MA5-15477_Cytokeratin_19_P08727-1_House_mouse.svg,antigenImageFileNamePDP:MA5-15477_Cytokeratin_19_P08727-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_10989877

Product Specific Information

MA5-15477 targets Cytokeratin 19 in indirect ELISA, WB applications and shows reactivity with Human samples.

The MA5-15477 immunogen is purified recombinant fragment of Cytokeratin 19 (aa80-400) expressed in E. Coli strain.
.

MA5-15477 detects Cytokeratin 19 which has a predicted molecular weight of approximately 44kDa.

Target Information

Cytokeratin 19 is part of a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight from 40-68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. For example, cytokeratin 19 is not expressed in hepatocytes, therefore, it is useful in the identification of liver metastasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.