Thermo Fisher Scientific F(ab`)2-Rabbit anti-Mouse IgG (H+L) Secondary Antibody
Rabbit에서 유래한 Polyclonal F(ab`)2 형식의 anti-Mouse IgG (H+L) 2차 항체. Western blot, IHC, ELISA, IP 등 다양한 실험에 사용 가능. 비결합(Unconjugated) 상태로 제공되며, 높은 특이성과 감도를 제공. 연구용으로만 사용.
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Applications 및 권장 희석 배수
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
| Immunoprecipitation (IP) | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.5 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2533935 |
| Target | IgG |
| Antibody Form | F(ab`)2 Fragment |
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins. These antibodies are useful in the detection, sorting, or purification of mouse immunoglobulins. Secondary antibodies offer increased versatility, enabling the use of various detection systems such as HRP, AP, or fluorescence. They also enhance sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Most secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species. They can be further purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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