Thermo Fisher Scientific HGF Polyclonal Antibody
HGF 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, Flow Cytometry에 사용 가능. Human 시료 반응성. KLH 접합 합성 펩타이드(521–554 aa) 면역원. 2.0 mg/mL 농도의 액상 형태로 제공되며, PBS(pH 7.4)에 0.09% sodium azide 포함. 연구용 전용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
| Flow Cytometry (Flow) | 1:10–1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide between 521–554 amino acids from the C-terminal region of human HGF |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.0 mg/mL |
| Purification | Ammonium sulfate precipitation, Size-exclusion - Dialysis |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2264123 |
Target Information
Hepatocyte growth factor (HGF) regulates cell growth, motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the c-Met receptor. It is secreted by mesenchymal cells and functions as a multifunctional cytokine primarily on epithelial cells. HGF promotes mitogenesis, cell motility, and matrix invasion, playing a central role in angiogenesis, tumorigenesis, and tissue regeneration.
HGF is secreted as a single inactive polypeptide and cleaved by serine proteases into a 69-kDa alpha-chain and a 34-kDa beta-chain, forming an active heterodimer via a disulfide bond. The protein belongs to the plasminogen subfamily of S1 peptidases but lacks protease activity. Alternative splicing generates multiple transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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