Thermo Fisher Scientific Citrulline Monoclonal Antibody (2D3.1), APC
Citrulline 잔기를 특이적으로 인식하는 마우스 단클론 항체로 APC 형광 표지 적용. Western blot, IHC, ICC/IF, ELISA 등 다양한 응용에 적합. 단백질 탈이미노화 연구용으로 사용. 연구용으로만 제공.
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Thermo Fisher Scientific Citrulline Monoclonal Antibody (2D3.1), APC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| ELISA | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2D3.1 |
| Immunogen | Synthetic L-Citrulline conjugated to Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage conditions | 4°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932207 |
Additional Formats
- Unconjugated (MA5-27573)
- FITC (MA5-45754)
- PE (MA5-45756)
- PerCP (MA5-45755)
- Request custom conjugation available
Product Specific Information
A 1:1,000 dilution of MA5-45753 was sufficient for detection of Citrulline in 2 µg of Citrulline conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for citrulline residues on modified proteins. Tested using citrulline conjugated Bovine Serum Albumin (BSA).
Target Information
Citrulline is an amino acid that is not incorporated into proteins during synthesis but is generated within proteins by the deimination of arginine. Citrulline occurs normally in many proteins, such as Myelin Basic Protein (MBP), while others, such as fibrin, become citrullinated during inflammation or apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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