Thermo Fisher Scientific Citrulline Monoclonal Antibody (2D3.1), FITC
Citrulline 잔기 검출용 FITC 결합 단클론 항체로, WB, IHC, ICC/IF, ELISA에 사용 가능. Mouse IgG1 기반으로 높은 특이성과 재현성을 제공. 단백질 내 Citrulline 변형 연구에 적합하며, 4°C 암소 보관.
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Thermo Fisher Scientific Citrulline Monoclonal Antibody (2D3.1), FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| ELISA | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2D3.1 |
| Immunogen | Synthetic L-Citrulline conjugated to Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932208 |
Additional Formats
- Unconjugated (MA5-27573)
- APC (MA5-45753)
- PE (MA5-45756)
- PerCP (MA5-45755)
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45754 was sufficient for detection of Citrulline in 2 µg of Citrulline conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for citrulline residues on modified proteins. Tested using citrulline conjugated Bovine Serum Albumin (BSA).
Target Information
Citrulline is an amino acid not incorporated into proteins during synthesis but generated within proteins by deimination of arginine. It occurs normally in many proteins such as Myelin basic protein (MBP), while others like fibrin become citrullinated during inflammation or apoptosis.
Usage Notice
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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