Thermo Fisher Scientific ELL Monoclonal Antibody (OTI3A8), TrueMAB
ELL 단백질을 인식하는 마우스 모노클로날 항체로, Western blot과 IHC(파라핀) 실험에 적합합니다. 고순도의 친화 크로마토그래피 정제 제품이며, 동결건조 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3A8 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 302–621 of human ELL produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
ELL is an elongation factor component of the super elongation complex (SEC), which enhances the catalytic rate of RNA polymerase II transcription by suppressing transient pausing at multiple DNA sites.
It is also part of the little elongation complex (LEC), regulating small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III.
ELL plays a critical role in stabilizing RNA polymerase II recruitment and early elongation prior to SEC assembly.
[UniProt: P55199-1]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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