Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), Functional Grade, eBioscience
인간 CD127(IL-7Rα)을 인식하는 eBioRDR5 클론의 기능 등급 단클론 항체입니다. Flow cytometry 및 기능 분석에 적합하며, IL-7 수용체 연구 및 T세포 조절 연구에 유용합니다. 고순도(>90%) 및 낮은 엔도톡신 수준으로 제공됩니다.
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4개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), Functional Grade, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Frozen) | - | 1 publication |
| Flow Cytometry | 0.06 µg/test | 19 publications |
| Functional Assay | Assay-Dependent | - |
| Control | Assay-Dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioRDR5 |
| Conjugate | Functional Grade |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage conditions | 4°C |
| Shipping conditions | Wet ice |
| RRID | AB_2865858 |
| Filtration | 0.2 µm post-manufacturing filtered |
| Purity | >90% (SDS-PAGE) |
| Endotoxin Level | <0.001 ng/µg antibody (LAL assay) |
| Aggregation | <10% (HPLC) |
Available Conjugates
- Unconjugated
- Alexa Fluor 488, 532, 561, 647, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 496, 563, 737, 805
- Brilliant Violet 421, 711, 786
- eFluor 450, 506, 660
- FITC
- NovaFluor Blue/Red/Violet/Yellow series
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Custom conjugation available
Product Specific Information
Description:
The eBioRDR5 monoclonal antibody reacts with human CD127 (Interleukin-7 Receptor alpha). CD127 complexes with CD132 (common gamma chain) to form the IL-7 receptor (IL-7R). CD127 is a type I glycoprotein (75–80 kDa) expressed by immature B cells, thymocytes, and most mature T cells, with transient down-regulation upon activation. IL-7 binding triggers Jak/STAT-mediated signaling essential for lymphocyte development and T-cell homeostasis.
CD127 expression is down-regulated on CD4⁺CD25⁺ regulatory T cells (Tregs). Cells co-expressing CD4 and CD25 but lacking CD127 are enriched for regulatory T cells expressing Foxp3 and exhibiting suppressive activity.
Binding of eBioRDR5 to PBMCs is blocked by pre-incubation with recombinant human IL-7 (Product #14-1079-80).
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on normal human peripheral blood cells at ≤0.06 µg/test (100 µL reaction). Cell number: 10⁵–10⁸ cells/test. Optimize titration for best performance.
Storage and Handling: Use in a sterile environment.
Target Information
CD127 (Interleukin-7, IL-7) is a glycoprotein involved in lymphopoiesis regulation. Its receptor (IL7R) is a heterodimer of alpha and gamma chains. The gamma(c) chain activates signal transduction, while the alpha chain mediates specific signaling events. CD127 promotes proliferation of precursor B cells, thymocytes, and mature T cells. Dysfunction is associated with severe combined immunodeficiency disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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