Thermo Fisher Scientific LC3A/LC3B Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide within Human LC3A aa 1-50 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:LC3A/LC3B,uniProtId:Q9GZQ8-1,ncbiNodeId:9606,antigenRange:1-50,antigenLength:125,antigenImageFileName:PA5-109226_LC3ALC3B_Q9GZQ8-1_Rabbit.svg,antigenImageFileNamePDP:PA5-109226_LC3ALC3B_Q9GZQ8-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:LC3A/LC3B,uniProtId:Q9H492-1,ncbiNodeId:9606,antigenRange:1-50,antigenLength:121,antigenImageFileName:PA5-109226_LC3ALC3B_Q9H492-1_Rabbit.svg,antigenImageFileNamePDP:PA5-109226_LC3ALC3B_Q9H492-1_Rabbit_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol, 0.2% BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2854637

Product Specific Information

Positive Control: HepG2, PC-3M, SH-SY5Y, rat brain tissue, mouse brain tissue, mouse heart tissue lysate, mouse cerebellum tissue.

Target Information

Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment. There are 16 proteins participating in the autophagy pathway in human. The autophagy protein LC3, a mammalian homologue of Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. LC3 is the only known mammalian protein identified that stably associates with the autophagosome membranes. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.