Thermo Fisher Scientific VHL Recombinant Rabbit Monoclonal Antibody (5A8A9)
VHL 단백질을 인식하는 토끼 유래 재조합 단클론 항체로, Western blot 및 ELISA에 최적화되었습니다. HEK293 세포에서 발현되었으며 Protein A로 정제되었습니다. 인간, 마우스, 랫트 시료에 반응하며, 고순도의 액상 형태로 제공됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 5A8A9 |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 100–200 of human Von Hippel Lindau (VHL) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS (pH 7.3), with 50% glycerol, 0.05% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
Product Specific Information
Immunogen Sequence:
TLPPGTGRRI HSYRGHLWLF RDAGTHDGLL VNQTELFVPS LNVDGQPIFA NITLPVYTLK ERCLQVVRSL VKPENYRRLD IVRSLYEDLE DHPNVQKDLE R
Target Information
Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to multiple malignant and benign tumors. A germline mutation of this gene underlies familial inheritance of VHL syndrome. The VHL protein forms a complex with elongin B, elongin C, and cullin-2, possessing ubiquitin ligase E3 activity. It mediates ubiquitination and degradation of hypoxia-inducible factor (HIF), a transcription factor central to oxygen-regulated gene expression. RNA polymerase II subunit POLR2G/RPB7 is also a reported target. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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