Thermo Fisher Scientific SCK Polyclonal Antibody
SHC2 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF에 사용 가능. Human과 Rat 반응성 확인. 액상 형태로 1 mg/mL 농도, PBS/glycerol buffer에 보관. VEGF 신호전달 관련 연구에 적합.
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Thermo Fisher Scientific SCK Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Reference |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human SHC2 (Accession P98077), amino acid residues A297–P347 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2854412 |
Product Specific Information
Antibody detects endogenous levels of total SHC2.
Target Information
Src homology 2 (SH2) domains bind specifically to tyrosine-phosphorylated proteins involved in signal transduction.
Shc-like protein (Sck) is a neuronal adaptor protein containing an N-terminal PTB domain, a collagen homology (CH) domain, and a conserved C-terminal SH2 domain.
Human Sck transcripts are highly expressed in liver, pancreas, prostate, and ovary.
In vascular endothelial cells, Sck participates in VEGF-induced signal transduction. Treatment of HUVEC cells with VEGF induces recruitment of Sck to tyrosine-1175 of KDR and enhances Sck tyrosine phosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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