Thermo Fisher Scientific GUK1 Polyclonal Antibody
Thermo Fisher Scientific의 GUK1 Polyclonal Antibody는 인간 GUK1 단백질을 인식하는 토끼 유래 다클론 항체로, Western blot 및 IHC(P)에서 검증되었습니다. 항원 친화 크로마토그래피로 정제되었으며, PBS와 글리세롤 버퍼에 보관됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:2,500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human GUK1. Recombinant protein control fragment (Product # RP-94310) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.4 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2791070 |
Product Specific Information
Immunogen sequence:
LRPIYISVQP PSLHVLEQRL RQRNTETEES LVKRLAAAQA DMESSKEPGL FDVVIINDSL DQAYAELKEA LSEEIKKAQR TGA
Target Information
Guanylate kinase (GK) catalyzes the ATP-dependent phosphorylation of GMP into GDP, essential for recycling GMP and indirectly cGMP. In prokaryotes, lower eukaryotes, and vertebrates, GK is a conserved monomeric protein (~200 amino acids). It shows structural similarity to protein A57R (SalG2R) from Vaccinia virus. Proteins containing DHR, SH3, and GK-like domains are termed MAGUKs (membrane-associated guanylate kinase homologs), including:
- Drosophila lethal(1)discs large-1 tumor suppressor (dlg1)
- Mammalian tight junction protein Zo-1
- Synaptic proteins interacting with NMDA receptor subunits (SAP90/PSD-95, CHAPSYN-110/PSD-93, SAP97/DLG1, SAP102)
- Vertebrate 55 kD erythrocyte membrane protein (p55)
- C. elegans protein lin-2
- Rat protein CASK
- Human proteins DLG2, DLG3
GK has an ATP-binding site (P-loop) in the N-terminal region, not conserved in GK-like domains of MAGUKs, though MP-binding residues are retained.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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