Invitrogen™

SuperScript™ IV RT-LAMP Master Mix

Invitrogen SuperScript IV RT-LAMP Master Mix is a ready-to-use solution optimized for fast, sensitive, and simple RNA target detection in자세히 알아보기

Invitrogen SuperScript IV RT-LAMP Master Mix is a ready-to-use solution optimized for fast, sensitive, and simple RNA target detection in loop-mediated isothermal amplification (LAMP) reactions. With this convenient master mix formulation, you can perform isothermal amplification from both RNA or DNA targets in a single tube format.

Benefits of SuperScript IV RT-LAMP Master Mix
• RNA and DNA target detection using Bst DNA polymerase for isothermal amplification in as little as five minutes
• Robust and specific cDNA synthesis using SuperScript IV Reverse Transcriptase
• Enhanced specificity and sensitivity with buffer formulation and RNaseOUT recombinant ribonuclease inhibitor
• Compatible with real-time and endpoint detection—includes SYTO 9 dye for real-time detection
• Flexible design provides several options for evaluating results, including real-time and endpoint detection methods

SuperScript IV RT-LAMP Master Mix includes an evolved Bst DNA polymerase mutant with a fast reaction speed and increased sensitivity and tolerance to inhibitors, as well as SuperScript IV Reverse Transcriptase with increased processivity, thermostability, and inhibitor resistance. To prevent RNA degradation by carry-over RNases, the master mix includes the potent, noncompetitive RNaseOUT Recombinant Ribonuclease Inhibitor. Our proprietary reaction buffer composition is optimized to increase specificity by delaying no-template control (NTC) amplification time.

사양

제품 유형Master Mix

용도(애플리케이션)LAMP, RT-LAMP

중합효소Bst DNA Polymerase

수량100 reactions, 1 x 1.25 mL

반응 수100 Reactions

최적 반응 온도65°C

역전사 효소SuperScript IV

배송 조건Dry Ice

검출 방법Real-time and end-point detection

라벨 또는 염료 포함SYTO 9 Green Fluorescent Nucleic Acid Stain

농도2X

반응 시간5 to 15 minutes

Unit SizeEach