Thermo Fisher Scientific PRPF8 Polyclonal Antibody
PRPF8 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체로, WB, IHC, IP 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 4°C에서 보관합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 2285 and 2335 of Human Pre-mRNA-Processing Factor 8 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
Pre-mRNA splicing occurs in two sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes and is essential for the catalytic step II in the pre-mRNA splicing process. It contains several WD repeats that function in protein–protein interactions. This protein shows sequence similarity to the yeast Prp8 protein and is a candidate gene for autosomal dominant retinitis pigmentosa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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