Thermo Fisher Scientific MUTYH Polyclonal Antibody
Thermo Fisher Scientific의 MUTYH Polyclonal Antibody는 인간 MUTYH 단백질을 인식하는 고품질 항체로, WB, IHC, ICC/IF 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 연구용으로만 사용됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20–1:50 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Protein corresponding to Human MUTYH. Recombinant protein control fragment (Product #RP-89477). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2856162 |
Product Specific Information
Immunogen sequence:
PDVEECAPNT GQCHLCLPPS EPWDQTLGVV NFPRKASRKP PREESSATCV LEQPGALGAQ ILLVQRPNSG LLAGLWEFPS VTWEPSEQLQ RKALLQELQR WA
Target Information
MYH (mutY homolog (E. coli)) is a DNA glycosylase mismatch repair enzyme that, in conjunction with mutM (OGG1), cleaves adenine residues paired with oxidized (8-hydroxyguanines) or non-modified guanines to correct A/G and A/C mismatches.
Repair of most modified and mispaired bases in the genome is initiated by DNA glycosylases, which bind and cleave N-glycosyl bonds to initiate base excision repair. MYH is crucial for avoiding mutations from oxidative DNA damage.
Multiple N-terminal splice variants of MYH exist in mammalian cells. Increasing levels of MYH in A549 cells exposed to oxygen and infrared radiation improve cell survival.
Biallelic MYH germ-line mutations predispose humans to colorectal adenomas and carcinomas. MYH is abundant in neurons, maintaining mitochondrial genome integrity against reactive oxygen species (ROS) throughout the mammalian lifespan.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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