Thermo Fisher Scientific MUTYH Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:50

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Protein corresonding to Human MUTYH. Recombinant protein control fragment (Product #RP-89477). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MUTYH,uniProtId:Q9UIF7-1,ncbiNodeId:9606,antigenRange:324-425,antigenLength:546,antigenImageFileName:PA5-110751_MUTYH_Q9UIF7-1_Rabbit.svg,antigenImageFileNamePDP:PA5-110751_MUTYH_Q9UIF7-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2856162

Product Specific Information

Immunogen sequence: PDVEECAPNT GQCHLCLPPS EPWDQTLGVV NFPRKASRKP PREESSATCV LEQPGALGAQ ILLVQRPNSG LLAGLWEFPS VTWEPSEQLQ RKALLQELQR WA

Target Information

MYH (mutY homolog (E. coli )) is a DNA glycosylase mismatch repair enzyme that in conjunction with mutM (OGG1), cleaves adenine residues paired with either oxidized (8-hydroxyguanines) or non-modified guanines in order to correct A/G and A/C mismatches. Repair of most modified and mispaired bases in the genome is initiated by DNA glycosylases, which bind and cleave N-glycosyl bonds to initiate base excision repair. MYH is crucial for the avoidance of mutations resulting from oxidative DNA damage. Multiple N-terminal splice variants of MYH exist in mammalian cells. Increasing levels of MYH in A549 cells exposed to oxygen and infrared radiation leads to improvements in cell survival. Biallelic MYH germ-line mutations predispose humans to colorectal adenomas and carcinomas. MYH is abundant in neurons where mitochondrial genomes exposed to reactive oxygen species (ROS) that damage DNA must maintain integrity over the entire mammalian life span.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.