Thermo Fisher Scientific Bromodeoxyuridine (BrdU) (Proliferation Marker) Monoclonal Antibody (85-2C8)
세포 증식 마커 BrdU를 검출하는 단클론 항체로, IHC, ICC, Flow Cytometry 등 다양한 응용 가능. Mouse IgG1, kappa 형식이며, Protein A/G로 정제된 액상 형태. 연구용으로 DNA 복제 중인 세포를 확인하는 데 사용.
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Thermo Fisher Scientific Bromodeoxyuridine (BrdU) (Proliferation Marker) Monoclonal Antibody (85-2C8)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chemical |
| Host/Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 85-2C8 |
| Immunogen | Bromodeoxyuridine (BrdU) conjugated to hemocyanine (isolated from Helix pomatia) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside and an analogue of thymidine. BrdU is widely used to detect proliferating cells in living tissues. It incorporates into newly synthesized DNA during the S phase of the cell cycle, substituting for thymidine during DNA replication. Antibodies specific for BrdU enable detection of incorporated BrdU, indicating cells actively replicating their DNA. Binding of the antibody requires DNA denaturation, typically achieved by acid or heat treatment.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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