Thermo Fisher Scientific HLA-A2 Monoclonal Antibody (BB7.2), NovaFluor Blue 585, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.6 µg)/test

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Product Specifications

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Additional Formats

• APC
• APC-eFluor 780
• NovaFluor Blue 510
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Yellow 570
• NovaFluor Yellow 660
• NovaFluor Yellow 730
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

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Product Specific Information

This BB7.2 monoclonal antibody recognizes the human major histocompatibility complex (MHC) class I molecule HLA-A2, a member of the HLA-A family. MHC class I consists of a single alpha chain with three domains, associated with beta 2-microglobulin, and is expressed on all nucleated cells.

The BB7.2 antibody exhibits blocking activity and also recognizes HLA-A28.

Each product includes one vial of NovaFluor conjugate and one vial of CellBlox Plus Blocking Buffer.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-diluted and tested by flow cytometry using normal human peripheral blood cells. Recommended use is 5 µL (0.6 µg) per test in a final volume of 100 µL. Cell number may range from 10⁵ to 10⁸ cells/test.

NovaFluor dyes are incompatible with DNA intercalating viability dyes such as propidium iodide, 7-AAD, and DAPI. Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.

This product ships with CellBlox Plus Blocking Buffer (Cat. No. C001T06F01), which improves staining performance. Add 5 µL per sample to antibody cocktails prior to combining with cells. For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology, based on nucleic acid dye structures engineered for optimized spectral performance with minimal spillover and spread.

Excitation: 494 nm
Emission: 585 nm
Laser: 488 nm (Blue)

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Target Information

HLA-A is a class I molecule consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain (~45 kDa) contains 8 exons:

• Exon 1: leader peptide
• Exons 2–3: α1 and α2 domains (peptide binding)
• Exon 4: α3 domain
• Exon 5: transmembrane region
• Exons 6–7: cytoplasmic tail

Polymorphisms in exons 2 and 3 determine peptide-binding specificity. HLA-A typing is routinely used in bone marrow and kidney transplantation. HLA/MHC antibodies are also key tools in immunopeptidomics for neoantigen identification via LC-MS/MS.

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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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