Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), NovaFluor Blue 690, eBioscience

Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), NovaFluor Blue 690

Applications and Tested Dilution

• Application: Flow Cytometry
• Tested Dilution: 0.4 µg/test

Product Specifications

Additional Formats Available

Unconjugated, Alexa Fluor 561, Alexa Fluor 660, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV/Violet/Yellow series, eFluor 450/506/660, FITC, PE series, PerCP-Cyanine5.5, Super Bright series, NovaFluor Blue/Red/UV/Violet/Yellow series, and custom conjugation available.

Product Specific Information

The eBio1D3 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein expressed on B cells during all developmental stages except plasma cells. Follicular dendritic cells also express CD19. Together with CD21, CD81, and MHC class II, CD19 forms a multimolecular complex associated with the BCR, mediating tyrosine phosphorylation, calcium flux, and B cell proliferation.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes; optimal use ≤0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Antibody should be titrated for best performance.

Compatibility Notice:
NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.

CellBlox Plus Blocking Buffer (Cat. No. C001T06F01):
Required for optimal staining with NovaFluor conjugates. Add 5 µL per sample (regardless of NovaFluor count) to antibody cocktails before combining with cells. For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology, based on engineered nucleic acid dye structures for optimized spectral performance minimizing spillover and spread.
Excitation: 494 nm; Emission: 585 nm; Laser: 488 nm (Blue).

Target Information

CD19 is a member of the immunoglobulin superfamily with two Ig-like domains, expressed on all peripheral B cells. It is also found on certain myeloid leukemia cells. CD19 acts as a signal-amplifying coreceptor for BCR and regulates B cell activation and proliferation. Mutations cause hypogammaglobulinemia; overexpression leads to B cell hyperactivity.

For Research Use Only. Not for diagnostic use or resale without authorization.

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