Thermo Fisher Scientific Phospho-S6 (Ser235) Polyclonal Antibody
S6 리보솜 단백질의 Ser235 인산화 부위를 인식하는 Rabbit polyclonal antibody. WB 및 ICC/IF에 사용 가능하며, Human, Mouse, Rat 반응성. 고순도 친화 크로마토그래피 정제, -20°C 보관, 연구용 전용.
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Thermo Fisher Scientific Phospho-S6 (Ser235) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around phosphorylation site of serine 235 (R-L-S(p)-S-L) derived from Human S6 Ribosomal Protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2554335 |
Product Specific Information
- Suggested positive control for Western blot: PC12 cells
- Suggested positive control for ICC/IF: HeLa cells
Target Information
Ribosomes are organelles that catalyze protein synthesis, consisting of a small 40S subunit and a large 60S subunit. These subunits include 4 RNA species and approximately 80 distinct proteins.
This gene encodes a cytoplasmic ribosomal protein, a component of the 40S subunit, belonging to the S6E family. It is the major substrate of protein kinases in the ribosome, with five C-terminal serine residues phosphorylated by different kinases. Phosphorylation is induced by growth factors, tumor-promoting agents, and mitogens, while dephosphorylation occurs at growth arrest.
The protein may regulate cell growth and proliferation through selective translation of specific mRNA classes. Multiple processed pseudogenes of this gene are dispersed throughout the genome.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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