Thermo Fisher Scientific ADAR1 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.

Target Information

Adenosine Deaminase RNA Specific (ADAR) catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA), a process known as A-to-I RNA editing. This modification can influence gene expression and protein function by altering codons, splice sites, RNA stability, and RNA structure-dependent processes such as microRNA production and protein-RNA interactions.

ADAR edits both viral and cellular RNAs, acting at multiple or specific sites. Cellular RNA substrates include BLCAP, GRIA2, HTR2C, and GABRA3, where editing leads to amino acid substitutions and functional changes.

Viral RNA substrates include HCV, VSV, MV, HDV, and HIV-1, showing either proviral or antiviral effects depending on the virus and mechanism (editing-dependent or independent). ADAR1 can inhibit HCV replication via RNA editing, while enhancing replication of MV, VSV, and HIV-1 through suppression of EIF2AK2/PKR activation. It also promotes HIV-1 infectivity by editing viral RNAs and modulates HDV replication via A-to-I editing at the amber/W site.

High levels of ADAR1 can inhibit HDV replication.
(Source: UniProt)

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.