Thermo Fisher Scientific KCNMA1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200

-

Product Specifications

Host/Isotype

Guinea pig / IgG

Class

Polyclonal

Type

Antibody

Immunogen

GST fusion protein corresponding to residues 1097-1196 of mouse KCNMA1variant 2 (Intracellular, C-terminus)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.8 mg/mL

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2857209

Product Specific Information

Reconstitute antibody with 50 µL of double distilled water (DDW). The reconstituted solution can be stored at 4 degrees C for up to 1 week. For longer periods, small aliquots should be stored at -20 degrees C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

GST fusion protein sequence is: SHSSHSSQS SSKKSSSVHS IPSTANRPNR PKSRESRDKQ NATRMTRMGQ AEKKWFTDEP DNAYPRNIQI KPMSTHMANQ INQYKSTSSL IPPIREVEDE C

Homology: Mouse - 21/21 amino acid residues identical to the canonical sequence (AccessionQ08460); rat - 20/21 amino acid residues identical to the canonical sequence (AccessionQ62976); human - 19/21 amino acid residues identical to the canonical sequence (AccessionQ12791).

Target Information

Potassium channels are a group of ubiquitously expressed proteins that serve numerous functions in excitable and non-excitable cells. One class of integral membrane potassium channels is the large conductance, calcium-activated potassium channel (Maxi K+). Maxi K+ differs from most other potassium channels in that its activation is controlled by both increases in intracellular calcium and by membrane depolarization. Maxi K+ dual activation is possible because of its structure. The core of the channel, which is similar to other potassium channels, is a Maxi K+ alpha homotetramer that contains both a voltage sensor and an intracellular calcium binding domain. In vascular smooth muscle, an auxiliary beta-subunit is found in a 1:1 stoichiometry. The beta-subunit exhibits its effect on the Maxi K+ channel by effectively decreasing by 5- to 10- fold the concentration of calcium required to keep the pore open.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.