LanthaScreen™ TR-FRET PPAR gamma Coactivator Assay Kit, goat

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15126:The LanthaScreen™ TR-FRET PPAR gamma Coactivator자세히 알아보기

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15126:

The LanthaScreen™ TR-FRET PPAR gamma Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential PPAR gamma ligands as agonists or antagonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a Peroxisome Proliferator Activated Receptor (PPAR) gamma ligand-binding domain (PPAR gamma-LBD) that is tagged with glutathione-S-transferase (GST), in a homogenous mix-and-read assay format.

*Agonist mode
*To run the LanthaScreen™ TR-FRET Peroxisome Proliferator Activated gamma Receptor Coactivator Assay in agonist mode (to identify agonist compounds), PPAR gamma-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-anti-GST antibody. After an incubation period at room temperature, the TR-FRET 520:495 emission ratio is calculated and used to determine the EC50 from a dose response curve of the compound. Based on the biology of the PPAR gamma-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).

*Antagonist mode
*When the LanthaScreen™ TR-FRET Peroxisome Proliferator Activated gamma Receptor Coactivator Assay is run in antagonist mode (to identify antagonist compounds), PPAR gamma-LBD is added to ligand test compounds followed by addition of a mixture of agonist, fluores-cein-coactivator peptide, and Tb-anti-GST antibody. The concentration of agonist used in this mode is the EC80 concentration as determined by first running the assay in agonist mode (Figure 2).

*Contents and Storage:
*The LanthaScreen™ TR-FRET PPARγ Coactivator Assay Kit contains PPAR gamma-LBD (GST) protein, fluorescently labeled TRAP220/ DRIP-2 coactivator peptide, Tb-anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).

사양

콘주게이트Tb (Terbium)

검출 방법Fluorescence

용도(장비)Microplate Reader

수량800 x 20 μL assays

포장384-well Plate

어세이 입력Biochemical coregulator interaction

유전자 ID(Entrez)5468

판독End Point

배송 조건Dry Ice

타겟 입력PPARG, PPAR gamma, NR1C3

제품라인LanthaScreen™

용도(애플리케이션)Co-Factor Interaction Assay, TR-FRET

리간드PPAR Gamma

제품 유형TR-FRET PPAR Gamma Coactivator Assay Kit

No. of Assays800 x 20 μL assays

Unit SizeEach