Thermo Fisher Scientific FICD Monoclonal Antibody (OTI15E10), TrueMAB
FICD 단백질을 인식하는 Mouse monoclonal antibody (Clone OTI15E10). Western blot 검증 완료. Human 시료 반응성. Lyophilized 형태로 제공되며, PBS buffer와 trehalose 함유. 연구용으로 ER 스트레스 및 단백질 AMPylation 연구에 적합.
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Applications
- Western Blot (WB): Tested dilution 1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI15E10 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 61–265 of human FICD produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
FICD is a dual-function enzyme capable of mediating both AMPylation and de-AMPylation of target proteins. The side chain of Glu-231 determines whether AMPylase or de-AMPylase activity occurs. It regulates the ERN1/IRE1-mediated unfolded protein response (UPR) by modifying HSPA5/BiP.
In unstressed cells, FICD AMPylates HSPA5/BiP at Thr-518, inactivating it. Under ER stress, it de-AMPylates the same residue, restoring activity. Although it can AMPylate RhoA, Rac, and Cdc42 in vitro, these are not physiological substrates.
(Source: UniProt)
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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