Thermo Fisher Scientific SPARC Polyclonal Antibody
SPARC 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ICC 등 다양한 응용에 사용 가능. 고순도 항원 친화 크로마토그래피 정제, 0.54 mg/mL 농도, 안정적 PBS/glycerol buffer 저장.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 1 publication |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | View publication |
| Immunocytochemistry (ICC/IF) | 1:100 | View publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human SPARC (exact sequence proprietary). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.54 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2736653 |
Product Specific Information
- Positive Control: NT2D1
- Predicted Reactivity: Pig (85%), Sheep (94%), Bovine (97%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
SPARC gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for collagen in bone to become calcified and is involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but also correlated with metastasis based on changes to cell shape promoting tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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